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Argentina are 6th in the group on 25 points - the same as Peru and one less than Chile and Colombia.
To qualify directly, Argentina must beat Ecuador and hope Chile and Colombia lose or draw their respective games against Brazil and Peru.
Two goals inside two minutes from Frickson Eraso and Espanyol striker Felipe Caicedo did the damage Looking calm and focused.
Sampaoli has tried out several variations over the past few days in training.