Method of validating analysing data asiandating com
The response factor method (determined by the analysis of standard materials or other methods prior to measurement of the analytes) is also used for calibration of the GC–FID.The FID response for hydrocarbons is generally proportional to the carbon number of the analyte .Statistical Tools for Method Development and Validation 5.Conclusion Acknowledgement References The variety of available techniques and analytical methods applied in laboratories calls for the need of defining the scope, range of application and quality of information obtained using each of them with reference to internationally agreed standards.
Some analytical methods include thin layer chromatography (TLC), high-performance liquid chromatography (HPLC) coupled with detectors such as refractive index (RI) detector, flame ionization detector (FID), density detector, evaporative light scattering detector (ELSD), and mass spectrometer detector.
Method development and validation of a process is needed to confirm that the analytical procedure employed for a specific test is suitable for its intended use.
It helps to improve the reliability, consistency and accuracy of analytical data : • Sample collection, sampling and labeling; • Sample transportation from field to laboratory and storage in appropriate conditions; • Sample preparation: extraction, drying, FAME preparation i.e.
Of these techniques, Gas Chromatography with Flame Ionization Detector (GC-FID) is most used in fatty acids analysis.
Method development and validation is useful for GC and other analytical procedures.Gas Chromatography seems to be the most appropriate method for qualitative and quantitative analysis of isolated lipid extracts.